src tyrosine kinase-specific inhibitor 4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (pp1)  (Millipore)

Journal:

Article Title: Transient alteration of T cell fine specificity by a strong primary stimulus correlates with T cell receptor down-regulation

doi:

Figure Lengend Snippet: Inhibition of protein tyrosine kinase activity blocks TCR down-regulation. P14 TCR transgenic spleen cells (A, B) or P14.G5 CTL clone (C, D) were preincubated for 60 min in the presence of PP1 or genistein. Preincubation with herbimycin A was done overnight with P14 spleen cells or for 60 min with P14.G5 CTL. EL-4 cells pulsed with p33 (1 μM) were then added for 4 h and cells stained for flow cytometry with anti-CD8 and anti-Vα2 antibodies (A, C) or with anti-CD8 and anti-CD69 antibodies (B, D). Mean fluorescence intensities are expressed as % or MFI of control in the absence of peptide.

Article Snippet: 4.13 Inhibition of src family kinase activity P14.G5 CTL (6×10 4 /well) or P14 spleen cells (5×10 5 /well) were preincubated for 60 min with the src family kinase-specific inhibitor PP1 (10 μM and 50 μM, respectively) (Bio-mol, Plymouth Meeting, PA) [ 28 ].

Techniques: Inhibition, Activity Assay, Transgenic Assay, Staining, Flow Cytometry, Fluorescence, Control